A Battery-Powered Notebook Thermal Cycler for Rapid Multiplex Real-Time PCR Analysis
نویسندگان
چکیده
منابع مشابه
A battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis.
A compact, real-time PCR instrument was developed for rapid, multiplex analysis of nucleic acids in an inexpensive, portable format. The instrument consists of a notebook computer, two reaction modules with integrated optics for four-color fluorescence detection, batteries, and a battery-charging system. The instrument weighs 3.3 kg, measures 26 x 22 x 7.5 cm, and can run continuously on the in...
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This paper presents a PC-based PCR thermal cycler with a host-local architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented host-local system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
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OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...
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The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2001
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac001709r